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KMID : 0613820060160030527
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Journal of Life Science
2006 Volume.16 No. 3 p.527 ~ p.533
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Improvement of Cultural Efficiency Using DNA Markers in Anther and Seed Culture of Rice
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Kim Kyung-Min
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Abstract
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The purpose of this study is to improve the culturability of a indica type rice cultivar, IR 36, using DNA marker associated with the ability of plant regeneration in anther and seed culture. The varietal difference of ability of callus formation and plant regeneration was investigated in anther and seed culture of 8 rice cultivars. Three japonica rice cultivars showed to have better culturability than those of tongil and indica type genotypes. But two indica/japonica lines, ’MGRI 079’ and ’MGRI 036’, which were selected to have good culturability in previous study showed the highest regenerability (20%) in anther culture of 8 rice cultivars. Thirty four BC₂F₄ lines were selected by marker screening using RZ400 for 100 BC₂F₄ lines derived from a cross ‘MGRI 079/IR 36*3’. The frequency of callus formation of 30 BC₂F₄ lines was higher than those of ‘IR 36’ in anther culture of the selected BC₂F₄ lines. The ability of plant regeneration of 15 lines was higher than that of ‘IR 36’ in the seed culture of 34 BC₂F₄ lines. A promising line, BC₂F₄-28, was selected to have better culturability in the anther and seed culture of the BC₂F₄ lines. The heading date and grain shape of the BC₂F₄-28 was similar to ‘IR 36’. In seed culture of 50 BC₄F₃ lines derived from a rice cross ’MGRI 079/IR 36*5’, 11 lines including BC₄F₃-3 showed to have higher regenerability compared with ’IR 36’. The highest frequency of plant regeneration (11%) was obtained from BC₄F₃-46 in seed culture of the BC₄F₃ lines.
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KEYWORD
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IR36, anther culture, MAS, culturabiltiy
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